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. Author manuscript; available in PMC: 2018 Aug 5.
Published in final edited form as: Nat Neurosci. 2018 Feb 5;21(3):324–328. doi: 10.1038/s41593-018-0074-8

Gene Primer sequence (5′ – 3′)

MrgprA1-F ATCCAGCAAGAGGAATGGGG
MrgprA1-R TGTGACCTAGGAGGAAGAAGAAG
MrgprA2-F CCTCCTACACAAGCCAGCAA
MrgprA2-R AAGCACAAGTGAAAGATGATGCT
MrgprA3-F GCTACATCCAGCAAGAGGAATG
MrgprA3-R GCAAAAATTCCTTTGGGTAGGGT
MrgprA4-F CCTGTGTGCTGTGATCTGGT
MrgprA4-R TCACGGTTAATCCAGGGCAC
MrgprA10-F CAGTGGTCCACCATCTCCAA
MrgprA10-R ACAGGCAAGAGAGTCATGGTT
MrgprA12-F TCAGGGATCGGGTGAAGCAC
MrgprA12-R GAGCATTTGAAGGTGTTGTTGGA
MrgprA14-F GGTTGCCCCTGTGTTTCTTC
MrgprA14-R TATTGCCAGTCAGTAAGCTGAG
MrgprA16-F GCCCTCTGGTTCCCATTACT
MrgprA16-R GTTTTTGGACCACTGAGGCATT
MrgprA19-F CAGGACCCAGATCACGACAC
MrgprA19-R TCCTGGGCTTCCGATTTCAC
MrgprB4-F TCTGGCTGGTGCTGATTTCTT
MrgprB4-R ACCACGAGGCTCAACAATAGA
MrgprB5-F CTGTGGTTCCTTCTGTGTCCA
MrgprB5-R TTTCCAGTTCCCCAGACCTTT
MrgprC11-F AGCATCCACAACCCCAGAAG
MrgprC11-R TGGAGTGCAGTTGGGATGAC
mACTB-F CGTCGACAACGGCTCCGGCATG
mACTB-R CCACCATCACACCCTGGTGCCTAGG
gpMrgprX1-F GCTGCCACTCATCCTGAGAA
gpMrgprX1-R ATCCACGCTCACAAACCCTT
gpTRPV1-F GGCCAATGGGGACTTCTTCA
gpTRPV1-R GAGTCCCTGGCACTGATGTC
gpACTB-F TGCTGCGTTACACCCTTTCT
gpACTB-R GCTCCAACCAACTGCTGTCA
hMrgprX1-F AGCGGCCGCCTTATATATTCC
hMrgprX1-R AGCTCAGGCCTGCAAAGTAG
hTRPV1-F GTGCTGTACTTCAGCCACCT
hTRPV1-R TGAAACGGCACAGGTCTCTC
hGAPDH-F TGGTCTCCTCTGACTTCAACAGCG
hGAPDH-R AGGGGTCTACATGGCAACTGTGAG