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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Neurotoxicology. 2018 Feb 20;65:166–173. doi: 10.1016/j.neuro.2018.02.012

FIGURE 1.

FIGURE 1

Mitochondrial-mediated stress elicited by the neurotoxin MPP+ in DAergic N27 cells results in increased association of PHD2 with both p23 and hsp90. Immunoprecipitation (IP) of PHD2 was performed using a monoclonal antibody specific for the PHD2 isoform followed by western blot analyses in cells treated with 500 μM MPP+ for 24 hrs versus untreated controls (C). Top left panel, PHD2 input; top middle panel, p23 western of PHD2 IP; top right panel; Hsp90 western of PHD2 IP. Below, densitometric quantitation of p23 and Hsp90 levels in Con versus MPP+ cells; #p<0.05 versus Con.