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. Author manuscript; available in PMC: 2019 Mar 1.

Published in final edited form as: Neurotoxicology. 2018 Feb 20;65:166–173. doi: 10.1016/j.neuro.2018.02.012

Administration of the pharmacological p23 inhibitor gedunin protects against MPP+ neurotoxicity in both N27 cells and in human iPSC-derived neurons. A, MPP+ levels were measured in whole cell lysates from N27 cell treated with 500 μM MPP⁺ alone and cells co-treated with MPP⁺ and Gedunin (1 and 5 μM) for 24 h via HPLC with electrochemical detection. MPP⁺ was extracted from cells homogenized in the presence of 0.4N perchloric acid and measured against a known standard. Values are presented as ng MPP+/mg protein, n = 3 per treatment condition. B, Cell viability in N27 cells treated with gedunin (5 μM, 24 hrs) versus untreated +/− MPP+. *p<0.01 versus untreated control; **p<0.05 versus MPP+ alone. C, iPSC-derived neurons exposed to gedunin (5 μM, 24 hrs) versus untreated +/− MPP+. *p<0.01 versus untreated control; **p<0.05 versus MPP+ alone. Cell viability was calculated via MTT assay in a minimum of three different wells and 4 separate fields in each well; *p<0.01 versus control; **p<0.05 versus MPP+. Values are presented as mean +/− SEM of % Con.

Administration of the pharmacological p23 inhibitor gedunin protects against MPP+ neurotoxicity in both N27 cells and in human iPSC-derived neurons. A, MPP+ levels were measured in whole cell lysates from N27 cell treated with 500 μM MPP⁺ alone and cells co-treated with MPP⁺ and Gedunin (1 and 5 μM) for 24 h via HPLC with electrochemical detection. MPP⁺ was extracted from cells homogenized in the presence of 0.4N perchloric acid and measured against a known standard. Values are presented as ng MPP+/mg protein, n = 3 per treatment condition. B, Cell viability in N27 cells treated with gedunin (5 μM, 24 hrs) versus untreated +/− MPP+. *p<0.01 versus untreated control; **p<0.05 versus MPP+ alone. C, iPSC-derived neurons exposed to gedunin (5 μM, 24 hrs) versus untreated +/− MPP+. *p<0.01 versus untreated control; **p<0.05 versus MPP+ alone. Cell viability was calculated via MTT assay in a minimum of three different wells and 4 separate fields in each well; *p<0.01 versus control; **p<0.05 versus MPP+. Values are presented as mean +/− SEM of % Con.