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. Author manuscript; available in PMC: 2018 Mar 18.
Published in final edited form as: Cell Syst. 2017 Nov 1;5(5):509–517.e3. doi: 10.1016/j.cels.2017.10.002

Figure 2. Increased constitutive expression of TetR shortens recovery, despite repressing TetA.

Figure 2

(A) We compare the native tet circuit to a synthetic strain where TetR is expressed from an inducible promoter and TetA is expressed from its native promoter. (B) Dynamic range of TetA expression provided by TetR regulation, obtained by constitutively expressing TetR at different levels, in the absence and in the presence of tetracycline. Green circles indicate the expression levels of the native circuit under the same conditions. (C) Gene expression in liquid cultures during exposure to 10μg/ml Tc, for low and high levels of constitutive expression of TetR. (D) TetA and TetR expression levels measured at the time of exposure for a range of TetR expressions. Points from (C) are indicated. (E) Delay in the recovery from drug exposure for the same cultures in (D), compared to the delay observed for the native circuit (green). (F) Growth curves for the points indicated in (C–E), showing a reduced delay in recovery for increased TetR expression. See also Figure S2.