Effect of glibenclamide, apamin, charybdotoxin, and tetraethylammonium chloride on MECN-induced antinociception in the acetic acid-induced abdominal constriction test in mice. Animals were pretreated with glibenclamide (GLIB; 10 mg/kg, i.p.), apamin (APA; 0.04 mg/kg, i.p.), charybdotoxin (CHAR; 0.02, i.p.), or tetraethylammonium chloride (TEA; 4 mg/kg, i.p.) 15 min before oral administration of either vehicle (10 mL/kg) or MECN (500 mg/kg). Each column represents the mean ± SEM of six mice. Statistical analyses were performed using 1-way ANOVA followed by Dunnett's post hoc test. ∗∗∗p < 0.001 compared to control group. ###p < 0.001 compared to 500 mg/kg MECN-treated group.