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. 2018 Mar 17;17:118. doi: 10.1186/s12936-018-2268-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — uRDT work flow. (1) 5 μL of sample is collected and (2) delivered to the sample well in the test cassette, followed by (3) the addition of four drops of assay buffer. (4) The test is allowed to incubate and the uRDT results are interpreted after 20 min while the SD-RDT results are interpreted after 15 min. The presence of a line of any intensity is considered for interpretation. (5) Tests with only a control line are interpreted as negative. (6) Tests with both control and test lines are interpreted as positive. (7) The absence of a control line indicates the test is invalid and should be repeated