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. Author manuscript; available in PMC: 2018 Mar 18.
Published in final edited form as: Nature. 2017 Jul 31;548(7668):466–470. doi: 10.1038/nature23470

Figure 1. Loss of NHEJ antagonizes DSB-induced inflammatory signaling.

Figure 1

a, Representative western blots for activation of STAT1 signaling at indicated times post-20Gy. NIR, non-irradiated. (n=3 biological replicates) b, Dose-dependency of STAT1 response at 6 days as in panel (a) (n=3 biological replicates). c, RT-qPCR of inflammatory gene induction following IR. Error bars represent SEM of 3 biological replicates. d, STAT1 activation monitored as in (a). Western blotting for STAT1 activation after 5h induction of DSBs by AsiSI or I-PpoI nuclease with Shield/4-OHT and followed by washout and recovery for the indicated times. e, CRISPR-Cas9 knockout of NHEJ components were monitored for STAT1 activation as in (a). f, Immunofluorescence of 53BP1 and γH2AX indicating residual DSBs in crNHEJ cells. Arrowhead indicates γH2AX positive micronucleus. Scale bar is 10µm.