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. Author manuscript; available in PMC: 2018 Mar 18.
Published in final edited form as: Nature. 2017 Jul 31;548(7668):466–470. doi: 10.1038/nature23470

Extended Data Figure 3. Loss of cGAS-STING signaling in micronuclei impedes IR-induced inflammatory gene activation with minimal impact on senescence.

Extended Data Figure 3

a, RT-qPCR of CCL5 and ISG56 at 6 days following 20Gy in parental MCF10A cells treated +/− CDK1i (RO-3306) or derivatives harboring deletion of cGAS or STING. Error bars represent SEM of three biological replicates. b, Knockouts for two separate CRISPR-Cas9 sgRNA for cGAS or STING cause similar reductions in STAT1 signaling. c, Senesence-associated β-galactosidase staining of 6D following indicated treatments. Error bars represent SEM of at least 2 biological replicates. d, Representative immunofluorescent staining of cGAS shows loss of staining in cGAS knockout cells. Scale bar is 10um. e, Immunofluorescent staining shows costaining of Lamin B2 in cGAS positive micronuclei. Scale bar is 10µm.