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. Author manuscript; available in PMC: 2018 Mar 18.
Published in final edited form as: Nature. 2017 Jul 31;548(7668):466–470. doi: 10.1038/nature23470

Extended Data Figure 5. cGAS-STING driven inflammatory signaling occurs in micronucleated cells.

Extended Data Figure 5

a, Representative images of pIFNβ-GFP reporter cells treated as indicated. Scale bar is 10µm. Quantification is as described in Methods and error bars represent SEM of 2 biological replicates. b, Mean nuclear intensity of pIRF3 staining was quantified. p-values are based on pooled data from 3 independent experiments and calculated by 1-way ANOVA. c, Representative western blot of STAT1 activation following transfection of herring testis DNA (HT-DNA) in indicated CRISPR-Cas9 knockout MCF10A cell lines shows STAT1 activation in DNA-PK and all NHEJ deficient cells, but not in cGAS STING knockouts.