Figure 10.
Demonstration of the depth-resolved capability of drOPD mapping. (A) Bright-field image and (B) transmission quantitative phase map of a 5μm section of HeLa cell block. (C–E) The corresponding drOPD maps of the 5μm section at the optical depth (zopl) of (C) 1μm, (D) 2μm and (E) 5μm. The dashed lines from the insets illustrate the location of the optical depth with respect to the sample where the thicker bottom layer indicates the glass slide that faces the incoming light. The pseudo color shown in (B) is the integrated optical path length along the axial direction and those in (C–E) are drOPD value. The colorbar represents values in nanometer.