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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Methods. 2017 Nov 7;136:134–151. doi: 10.1016/j.ymeth.2017.10.011

Figure 7.

Figure 7

(A) Squared gradient plot versus the axial position of the objective lens at the wavelength of 550 nm. (B) The dependence of the focal-plane shift on the wavelength. Each point was the average value from 20 measurements (orange line), and the error bar indicates standard deviation. Data was then fitted to a fourth-order polynomial (blue line).