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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Ticks Tick Borne Dis. 2017 Dec 24;9(3):443–449. doi: 10.1016/j.ttbdis.2017.12.013

Figure 3.

Figure 3

Enzyme activity assays of GST-FolA of Rickettsia species phylotype G021. The dihydrofolate reductase activity of the GST-FolA protein was monitored at 25ºC for 10 min. The absorbance change at 340 nm was observed using a SpectraMax i3x plate reader. 3A) The graph represents the FolA specific activity assay. 150 μg GST-FolA or GST-GltA protein was used in the enzymatic assay. GST-GltA and buffer only serve as two controls for the assay. 3B) This graph represents the determination of the optimum enzyme concentration for the specific activity assay. 125 μg, 150 μg, and 175 μg GST-FolA were used in the enzymatic assay.