Figure 2.

AdNRTN Treatment Increases Parasympathetic Innervation of Ex Vivo SMG Culture after Radiation

(A) Whole-mount image of a SMGs cultured for 72 hr after IR. The SMGs were immunostaining for nerves (Tubb3), NRTN, and perlecan, which stain the basement membrane. The area in the white box is shown in (B). (B) Higher magnification of terminal endbuds and nerves. Individual endbud cells with increased NRTN expression are apparent with AdNRTN treatment (white arrows). Scale bars: 250 μm (A); 50 μm (B).