Figure 5.

Treatment of SMGs In Vivo with Increasing Doses of AdNRTN Increases Nrtn Transcripts within the Gland and Improves Some Neuronal Markers after IR

(A) qPCR analysis of Nrtn transcripts in SMGs 60 days after IR and treatment with increasing doses of AdNRTN. (B) Additional qPCR of genes related to NRTN signaling in IR SMGs treated with AdNRTN 10⁹ compared with IR treatment alone. The expression of the NRTN receptors Gfra2 and Ret kinase, neuronal markers Tubb3, and the parasympathetic markers Chat and Vacht and the sympathetic markers Th and Adra2b. Gene expression was normalized to non-IR SMGs and the ribosomal housekeeping gene Rps29 (data not shown). qPCRs were done in triplicate, and N = 11–12 SMGs analyzed. ^#Vacht expression was not detected in 5 of 11 SMGs. Error bars represent the SEM. ANOVA with post hoc Dunnett’s test compared with non-IR, *p < 0.05; **p < 0.01; ***p < 0.001.