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Study design and sample collection. Groups of C57BL/6 mice were subcutaneously primed with H56 alone (H56) or combined with CAF01, and boosted at day 28 with H56 + CAF01, or H56 + o/w squalene or H56 alone. Blood samples were collected at day 0, 12, 28, 31, and 38 following priming; draining lymph nodes (dLN) and spleens (Spl) were collected at day 7, 28, 31, and 38. The abbreviations of the different prime-boost combinations are here reported and used in all the figures.
