Figure 5.

Enhancement of dopamine (DA) release by actin filament disruption. PC12 cells were incubated for 2 h in the presence or absence (○) of 3 μM cytochalasin D (●) or 1 μM latrunculin A (■) under culture conditions. After the incubation, the cells were washed three times with low-K⁺ solution, and then the extracellular buffer was changed four times every 2 min. Dopamine release was evoked with 0.5 μM ionomycin. The amount of dopamine released into the medium was determined and expressed as a percentage of the total cellular content. The values are the mean ± SEM from four representative experiments.