Fig. 3.

BCAAs prevent ER stress in cultured cells. (A–C) Western blot analysis of HeLa (A and C) and 661W (B) cells. (A and C) HeLa cells were cultured with TM (3 μg/mL) with or without BCAAs for 6 hours. (B) 661W cells were cultured with oligomycin (1 μg/mL) with or without BCAAs for 24 hours. (A and B) C/EBP-homologous protein (CHOP), (C) phosphorylated mammalian target of rapamycin (pmTOR, phosphorylated at S2481) and phosphorylated translational suppressor eIF4E binding protein-1 (p4E-BP1) were analyzed. Actin was used for a loading control. Complete scans of western blots are shown in Fig. S4A−C.