. 2018 Mar 1;4(2):e00549. doi: 10.1016/j.heliyon.2018.e00549

Table 1.

Primer sets used for standardization of the assay.

Set	Primer Orientation	Primer Name	Primer position	Primer Sequence	Product Size	Reference
Set 1	Forward	KFD_9090_F	9090–9110	CCGAGAAGCAGTGGAGGACC	260bp	Designed during this study
Set 1	Reverse	KFD_9350_R	9324–9350	GCCATCCCAGGTAGTTAAGACTGGTC	260bp
Set 2	Forward	KFD_8610_F	8610–8633	ACTGGCACAGCGTGTGTGGTACT	330bp
Set 2	Reverse	KFD_8940_R	8918–8940	CCCTCATGATGATCTTGGTTCC	330bp
Set 3	Forward	KFD_7800_F	7800–7818	CGCAGAGGAGGTGCCGAG	360bp
Set 3	Reverse	KFD_8160_R	8141–8160	AGTCTTGGGACCTCATGGCC	360bp
Real time RT-PCR primers	Forward	KFD NS5F	548–567	TGGAAGCCTGGCTGAAAGAG	63bp	Mourya et al 2012 [12]
	Reverse	KFD NS5R	611–592	TCATCCCCACTGACCAGCAT
	Probe	KFD NS 5P	569–590	ATGGAGAGGAGCGCCTGACCCG
Nested RT-PCR	Outer forward	KFDNS5 3S	8896–8917	GTCAGATGAACAAAATCGCTGG	756bp	Mourya et al 2012 [12]
	Outer reverse	KFDNS5 3R	9632–9651	TCATCCCCACTGACCAGCAT	756bp
	Inner forward	KFDNS5 4S	9217–9235	GAAGAAGCTGTCCGAACTC	355bp
	Inner reverse	KFDNS5 4R	9554–9572	GGTCCTGTGAGTCAGATGG	355bp