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. 2018 Feb 26;200(7):2263–2279. doi: 10.4049/jimmunol.1700242

FIGURE 1.

FIGURE 1.

Study approach. Cell samples were stained in parallel using standard and optimized pMHC multimer staining protocols. The standard approaches used pMHC tetramer or dextramer, whereas the optimized protocol also included the PKI dasatinib and an anti-fluorochrome Ab. Multimer+ T cells were sorted by flow cytometry for TCR sequencing or cell cloning.

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