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. 2018 Mar 6;4(3):e00561. doi: 10.1016/j.heliyon.2018.e00561

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Detection of Hepatitis B Virus (HBV) from heat-treated blood samples using the Genie III system (OptiGene, UK). (A) Electrophoresis result of LAMP to blood heat-treatment. (B) Amplification plots of the LAMP assays from blood heat-treatment. (C) Electrophoresis results of HBV-DNA from blood samples using PCR method with F3 and B3 primers. Lane M = 1 kp marker; Lanes 1–7 = HCM.F.37, HCM.F.32.1, HCM.F.62, HCM.M.41, HCM.F.29, HCM.F.32.2, HCM.F.28 DNA-HBV samples; Lane 8 = Negative LAMP assay.