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. 2018 Mar 12;9:454. doi: 10.3389/fimmu.2018.00454

Figure 2.

Figure 2

Expression of the recognition and activation molecules of total monocytes and subsets. Expression of toll-like receptors (TLR) (A), and HLA-DR (B) by total monocytes, classical (CD14++CD16), intermediate (CD14++CD16+), and non-classical (CD14+CD16++) monocytes subsets in cultures without stimulation (control) and after in vitro stimulation with T. cruzi antigens (TRIPO). The groups evaluated were non-infected (NI) individuals (n = 6), indeterminate (IND, n = 10), and cardiac (CARD, n = 9) clinical forms of Chagas disease. Significant differences (p < 0.05) between groups are evidenced by lines and asterisks (*) according Kruskal–Wallis test, followed by Dunn’s post hoc test. The # sign indicates significant difference between control and TRIPO cultures using Wilcoxon signed-rank test. Boxes show the median and interquartile ranges, whiskers indicate the highest and lowest observation, and dots represent the outliers. MFI, mean fluorescence intensity.