Table 1.
Linear regression and correlation analysis of CD80 and CD86 co-stimulatory molecules by monocytes and their subsets with CD28 and CTLA-4 molecules and Th1 Th2, Th17, and regulatory T (Treg) cells.
| Total monocytes | Classical monocytes | Intermediate monocytes | Non-classical monocytes | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| R2 (p-value) | ρ (p-value) | R2 (p-value) | ρ (p-value) | R2 (p-value) | ρ (p-value) | R2 (p-value) | ρ (p-value) | |||
| Non-infected | CD80 | |||||||||
| CD28 | 0.73 (0.03)* | −0.03 (0.96) | 0.20 (0.38) | −0.09 (0.87) | 0.84 (0.01)* | −0.37 (0.47) | 0.001 (0.94) | 0.23 (0.66) | ||
| CTLA-4 | 0.58 (0.08) | 0.93 (0.008)* | 0.002 (0.93) | 0.20 (0.70) | 0.28 (0.28) | 0.43 (0.39) | 0.16 (0.43) | 0.03 (0.96) | ||
| Th1 | 0.07 (0.62) | 0.086 (0.87) | 0.21 (0.34) | −0.43 (0.40) | 0.04 (0.70) | 0.26 (0.63) | 0.18 (0.40) | 0.37 (0.47) | ||
| Th2 | 0.05 (0.68) | −0.54 (0.27) | 0.19 (0.38) | −0.43 (0.40) | 0.01 (0.88) | 0.09 (0.87) | 0.03 (0.76) | 0.029 (0.96) | ||
| Th17 | 0.04 (0.70) | 0.029 (0.96) | 0.36 (0.21) | −0.77 (0.07) | 0.03 (0.75) | 0.09 (0.87) | 0.13 (0.48) | 0.26 (0.63) | ||
| Treg | 0.33 (0.23) | −0.94 (0.00)* | 0.27 (0.29) | 0.2 (0.70) | 0.20 (0.38) | − 0.31 (0.54) | 0.01 (0.89) | − 0.06 (0.87) | ||
| CD86 | ||||||||||
| CD28 | 0.01 (0.84) | 0.03 (0.96) | 0.22 (0.34) | −0.64 (0.17) | 0.38 (0.19) | −0.54 (0.27) | 0.005 (0.89) | −0.26 (0.62) | ||
| CTLA−4 | 0.06 (0.65) | −0.26 (0.62) | 0.48 (0.13) | −0.14 (0.79) | 0.04 (0.71) | −0.23 (0.66) | 0.03 (0.76) | −0.20 (0.70) | ||
| Th1 | 0.27 (0.29) | 0.43 (0.40) | 0.02 (0.78) | −0.2 (0.7) | 0.02 (0.80) | 0.14 (0.79) | 0.60 (0.07) | 0.49 (0.33) | ||
| Th2 | 0.45 (0.14) | 0.43 (0.40) | 0.41 (0.17) | 0.54 (0.27) | 0.04 (0.71) | 0.43 (0.40) | 0.56 (0.09) | 0.54 (0.27) | ||
| Th17 | 0.04 (0.71) | 0.086 (0.87) | 0.048 (0.68) | −0.25 (0.62) | 0.01 (0.86) | 0.31 (0.54) | 0.64 (0.06) | −0.14 (0.79) | ||
| Treg | 0.22 (0.35) | 0.66 (0.16) | 0.43 (0.16) | 0.77 (0.07) | 0.01 (0.86) | −0.08 (0.87) | 0.79 (0.02)* | 0.6 (0.21) | ||
| Indeterminate | CD80 | |||||||||
| CD28 | 0.24 (0.18) | −0.58 (0.10) | 0.20 (0.23) | −0.57 (0.11) | 0.009 (0.78) | −0.18 (0.64) | 0.004 (0.87) | 0.07 (0.86) | ||
| CTLA−4 | 0.13 (0.39) | 0.40 (0.32) | 0.13 (0.38) | 0.29 (0.49) | 0.57 (0.03)* | 0.86 (0.007)* | 0.19 (0.29) | −0.31 (0.46) | ||
| Th1 | 0.10 (0.40) | 0.23 (0.56) | 0.03 (0.66) | 0.06 (0.88) | 0.01 (0.91) | 0.17 (0.67) | 0.08 (0.45) | 0.19 (0.62) | ||
| Th2 | 0.28 (0.14) | 0.47 (0.2) | 0.41 (0.06) | 0.75 (0.02)* | 0.19 (0.23) | 0.46 (0.22) | 0.27 (0.15) | −0.59 (0.09) | ||
| Th17 | 0.31 (0.12) | 0.39 (0.29) | 0.26 (0.16) | 0.40 (0.28) | 0.23 (0.18) | 0.40 (0.28) | 0.06 (0.54) | −0.06 (0.88) | ||
| Treg | 0.10 (0.42) | −0.73 (0.02)* | 0.07 (0.49) | −0.63 (0.07) | 0.00 (0.98) | −0.38 (0.30) | 0.02 (0.72) | 0.59 (0.09) | ||
| CD86 | ||||||||||
| CD28 | 0.16 (0.29) | −0.42 (0.26) | 0.003 (0.88) | −0.08 (0.83) | 0.07 (0.49) | −0.23 (0.55) | 0.10 (0.41) | −0.32 (0.41) | ||
| CTLA−4 | 0.17 (0.30) | 0.60 (0.12) | 0.55 (0.04)* | 0.67 (0.07) | 0.10 (0.45) | 0.29 (0.49) | 0.10 (0.44) | 0.45 (0.26) | ||
| Th1 | 0.17 (0.26) | 0.13 (0.75) | 0.004 (0.87) | 0.17 (0.67) | 0.04 (0.60) | −0.08 (0.83) | 0.15 (0.31) | 0.18 (0.64) | ||
| Th2 | 0.27 (0.26) | 0.54 (0.13) | 0.08 (0.46) | 0.24 (0.54) | 0.26 (0.16) | 0.41 (0.28) | 0.33 (0.10) | 0.68 (0.04)* | ||
| Th17 | 0.30 (0.13) | 0.29 (0.44) | 0.03 (0.67) | 0.21 (0.59) | 0.38 (0.08) | 0.49 (0.18) | 0.29 (0.13) | 0.24 (0.53) | ||
| Treg | 0.11 (0.38) | 0.66 (0.05) | 0.22 (0.20) | −0.78 (0.01)* | 0.12 (0.36) | −0.53 (0.14) | 0.10 (0.40) | −0.73 (0.03)* | ||
| Cardiac | CD80 | |||||||||
| CD28 | 0.003 (0.89) | 0.06 (0.86) | 0.003 (0.89) | 0.07 (0.87) | 0.16 (0.29) | 0.60 (0.09) | <0.01 (0.10) | −0.55 (0.13) | ||
| CTLA-4 | 0.05 (0.55) | −0.09 (0.81) | 0.05 (0.55) | −0.09 (0.81) | 0.10 (0.41) | −0.44 (0.24) | 0.38 (0.08) | −0.80 (0.00)* | ||
| Th1 | 0.02 (0.69) | −0.37 (0.33) | 0.02 (0.68) | −0.37 (0.33) | 0.03 (0.64) | −0.35 (0.36) | 0.004 (0.86) | −0.43 (0.24) | ||
| Th2 | 0.04 (0.59) | 0.20 (0.60) | 0.04 (0.59) | 0.20 (0.60) | 0.18 (0.25) | −0.33 (0.38) | 0.06 (0.52) | −0.20 (0.62) | ||
| Th17 | 0.0003 (0.96) | 0.13 (0.73) | 0.0003 (0.96) | 0.13 (0.73) | 0.09 (0.42) | −0.22 (0.58) | 0.008 (0.81) | −0.27 (0.49) | ||
| Treg | 0.33 (0.11) | 0.27 (0.49) | 0.33 (0.11) | 0.27 (0.48) | 0.17 (0.28) | −0.55 (0.12) | 0.001 (0.93) | 0.02 (0.97) | ||
| CD86 | ||||||||||
| CD28 | 0.18 (0.25) | −0.50 (0.17) | 0.18 (0.25) | −0.50 (0.17) | 0.03 (0.64) | −0.02 (0.97) | 0.23 (0.19) | 0.18 (0.64) | ||
| CTLA−4 | 0.81 (0.001)* | 0.61 (0.08) | 0.81 (0.001)* | 0.60 (0.08) | 0.02 (0.72) | 0.29 (0.46) | 0.54 (0.02)* | 0.54 (0.13) | ||
| Th1 | 0.02 (0.69) | 0.33 (0.38) | 0.02 (0.69) | 0.33 (0.38) | 0.07 (0.48) | 0.20 (0.60) | 0.15 (0.31) | 0.37 (0.33) | ||
| Th2 | 0.33 (0.10) | 0.44 (0.23) | 0.33 (0.10) | 0.44 (0.23) | 0.09 (0.43) | −0.38 (0.32) | 0.33 (0.10) | 0.02 (0.97) | ||
| Th17 | 0.001 (0.91) | 0.30 (0.43) | 0.001 (0.91) | 0.30 (0.43) | 0.11 (0.39) | −0.40 (0.29) | 0.29 (0.13) | 0.02 (0.97) | ||
| Treg | 0.005 (0.84) | 0.52 (0.15) | 0.005 (0.84) | 0.51 (0.15) | 0.04 (0.59) | −0.13 (0.73) | 0.10 (0.39) | 0.53 (0.14) | ||
Treg, T regulatory lymphocytes; data are presented by R-squared (R2) and Spearman’s coefficient (ρ), and respective p-value. Bold, gray background and asterisks (*) indicates a signifficant difference (p < 0.05).