Figure 3.

Identification of Compounds Reducing P525L FUS-eGFP SG Area in SL iPSCs

(A) Results for ∼1,000 compounds on P525L FUS-eGFP SG area arranged in ascending order. Compounds were considered as hits when Z < −3.

(B–D) Validation of selected compounds at 5 and 10 μM for their effects on P525L FUS-eGFP SG area (B). Each time, images were acquired from at least six positions/well from three different wells per line. Error bars indicate SEM. ^∗∗∗∗p < 0.0001. Example micrographs are shown for DMSO control (C) and selected hit compounds (D). Chemical structures for the selected compounds were downloaded from ChemSpider. Scale bar, 10 μm.