Figure 5.

Induction of Autophagy Rescues P525L FUS-eGFP SG Pathology in iPSCs

(A) Confocal micrographs of WT and P525L FUS-eGFP SL iPSCs treated with 15 μM rapamycin for 5 hr. Arsenite was added for the final 1 hr. LysoTracker red labels lysosomes, LC3 immunostaining highlights autophagosomes. Scale bar, 10 μm.

(B) Two hours of rapamycin increases LC3 puncta per cell relative to DMSO.

(C) Analysis of the frequency of LC3-positive FUS-eGFP SGs after 5 hr of rapamycin.

(D) Increased frequency of LTR-positive puncta per cell upon rapamycin treatment for 2 hr relative to DMSO.

(E) Analysis of the frequency of LTR-positive FUS-eGFP SGs after rapamycin treatment for 5 hr. All experiments used arsenite for 1 hr. n = 3 independent experiments, and images were acquired from at least six positions/well from four different wells per line.

Error bars indicate SEM. ^∗p < 0.05, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001.