Identification of Drugs for Repurposing
(A–D) Chart of Z scores from drug screen identifying US FDA-approved drugs able to reduce SG area (A). Representative images are shown for DMSO control in (B), sirolimus in (C), and selected anti-depressants and anti-psychotics in (D). Scale bars, 10 μm.
(E) SL P525L FUS-eGFP neurons treated with chlorpromazine, paroxetine, promethazine, and trimipramine show a slight reduction in FUS SG intensity. Error bars represent SEM.
(F and G) LL P525L FUS-eGFP neurons treated with the indicated compounds show a reduction in FUS SG intensity (F), which is significant for paroxetine, promethazine, rapamycin, and torkinib as displayed in the respective confocal micrographs (G). Scale bar, 10 μm. n = 3 independent experiments and in each experiment, images were acquired from three positions/well from three different wells per line. Error bars represent SEM. ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.