Fig. 5.

Infection rate of HIV-1 strains HE #0, HE #10 and NPO3, and HIV-2 strain EHO in U87.CD4.CXCR7 cells. Quantification of the % infection resulting from incubation of U87.CD4.CXCR7 cells with HIV-1 HE #0 (100,000 pg), HE #10 (5,000 pg), NPO3 (20,000 pg) and HIV-2 EHO (5,000 pg) was performed via measurement of the HIV p24/p27 capsid protein transcribed by the HIV replication machinery using an intracellular mAb staining against p24/p27. The data were obtained using flow cytometry (each dot plot represents % of infected cells for a given HIV strain). A representative staining was shown from one out of three independent experiments, all resulting in comparable infection rates for the given HIV strains. NI: non-infected.