Fig. 6.

DNA agarose gel showing HIV entry PCR on HIV LTR R/U5 and beta-actin DNA isolated from HIV-1 HE #10 infected U87.CD4.CXCR7 cells. The effect of the CXCR7 inhibitors is shown on U87.CD4.CXCR7 cells infected with 5,000 pg of HIV-1 HE #10. In brief, CD4/CXCR7-positive U87 cells were left untreated or were initially preincubated with the CXCR7 inhibitors 10D1-J16 mAb (10 μg ml⁻¹), CCX771 (5 μM) and CXCL11 (1 μM). Thereafter, all experimental conditions, except for the non-infected control, were infected with 5,000 pg HIV-1 HE #10. After a 2 h incubation period at 37 °C, 5% CO₂ the cells in each well of an experimental condition were lysed and total DNA was isolated. Finally, an HIV-1 specific PCR was performed on each isolated total DNA sample to check for the amount of proviral DNA. The PCR-amplified proviral DNA from the isolated total DNA samples was eventually run and visualized on a 2.2% agarose gel. A full, non-adjusted image of the DNA agarose gel is provided in the Supplementary content (Supplementary File 2).