Table 1.
Animals |
Food intake/exercise/treatment |
Age at time of sacrifice |
Processing of tissue |
Data |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Strain | Sex | Diet | Fat composition | Age at start diet (weeks) | AL | CR | RW | LPS | 6 m | 9 m | 12 m | 24 m | Snap frozen | 4% PFA fixed | FACS-sorting | Assay | Figs |
C57BL/6 OlaHsd, Harlan | Male | HFD | 42% fat, ab Diets® 4031.09 | 4 | + | + | + | + | IHC | 5 | |||||||
HFD | + | + | + | + | IHC | 5 | |||||||||||
HFD | + | + | + | + | IHC | 5 | |||||||||||
HFD | + | + | + | + | IHC | 5 | |||||||||||
LFD | 6.5% fat, ab Diets® 2141 AM-II | 4 | + | + | + | + | IHC | 5 | |||||||||
LFD | + | + | + | + | IHC | 5 | |||||||||||
LFD | + | + | + | + | IHC | 5 | |||||||||||
LFD | + | + | + | + | IHC | 5 | |||||||||||
C57BL/6J, Charles River | Male | HFD | 45% fat, Diets® D12451 | 12 | + | + | + | + | + | IHC | 1 | ||||||
LFD | 10% fat, Diets® D12450H | 6 | + | + | + | + | + | IHC | 1 | ||||||||
C57BL/6J, Charles River | Male | HFD | 60% fat, Diets® D12492 | 12 | + | + | + | + | qPCR | 2, 3 | |||||||
HFD | + | + | + | qPCR | 2, 3 | ||||||||||||
LFD | 10% fat, Diets® D12450B | 6 | + | + | + | + | qPCR | 2, 3 | |||||||||
LFD | + | + | + | qPCR | 2, 3 |
HFD, high fat diet; LFD, low fat diet; AL, ad libitum; RW, running wheel; CR, caloric restriction; IHC, immunohistochemistry; PFA, paraformaldehyde.