Figure 7. Effect of HDGF gene-silencing on the constitutive cytokine production in rat aortic smooth muscle cells (SMCs).

Cultured SMCs received scramble nucleotides or HDGF siRNA for 24 h, followed by another 24-h incubation. (A) Western blotting data confirmed gene-silencing efficiency of siNRA-mediated HDGF gene knockdown after 48-h treatment. Twenty-four hours of conditioned media after HDGF gene knockdown were subjected to ELISA, revealing that HDGF gene modification did not affect TNF-α release (B), but significantly reduced IL-6 production (C) in SMCs. Data are shown in mean ± SEM; *P<0.05 compared between groups; NS, not significant.