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. 2018 Mar 16;38(2):BSR20171014. doi: 10.1042/BSR20171014

Figure 1. HG induced the proliferation and migration of NSCLC cells through GAS5.

Figure 1

NSCLC cell lines (PC-9 and H1299) were cultured in LG (5 mM) and HG (25 mM) medium for 24 h, respectively. (A) The proliferation and (B) migration of NSCLC cells were detected using CCK-8 and Transwell assays, respectively. (C) The expression of GAS5 in NSCLC cell lines was determined by qRT-PCR, the levels of TRIB3 protein were measured using Western blot. NSCLC cells were transfected with pcDNA-GAS5 and then cultured with HG medium. (D) The expression of GAS5 in NSCLC cells was up-regulated by pcDNA-GAS5. GAS5 overexpression obviously suppressed the (E) proliferation and (F) migration of NSCLC cells treated with HG. For (A–D), *P<0.05 compared with LG or HG + pcDNA; for (E,F),*P<0.05 compared with LG,#P<0.05 compared with HG + pcDNA.