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. 2018 Mar 16;38(2):BSR20171014. doi: 10.1042/BSR20171014

Figure 2. The interaction of GAS5 and TRIB3 in NSCLC cells.

Figure 2

NSCLC cells were divided into four experimental groups: LG, HG, HG + pcDNA (transfection of pcDNA and HG treatment) and HG + pcDNA-GAS5 (transfection of pcDNA-GAS5 and HG treatment). (A) The levels of TRIB3 protein were measured using Western blot; *P<0.05 compared with LG; #P<0.05 compared with HG + pcDNA. (B) RNA pull-down assays revealed that TRIB3 protein was presented in the complex pull-down by GAS5. NS was the negative control for GAS5 and NC was the same RNA as GAS5, but it was without biotin label. (C) RIP assays confirmed that GAS5 was enriched in the protein–RNA complex precipatated by TRIB3 from NSCLC cells; #P<0.05 compared with NC.