Figure 2. The impact of heterologous expression of PPIP5K1 upon PP-InsP turnover in Drosophila S3 cells.

(A) Western analysis of PPIP5K1 expression in S3^vector and S3^PPIP5K1 cells. (B) Confocal immunofluorescence analysis of PPIP5K1 distribution and Hoechst staining in S3^PPIP5K1 cells (left panel) or S3^vector cells (right panel); white curved lines highlight a tendency for the PPIP5K1 to be more concentrated in punctae near the plasma membrane; scale bars = 5 μm. (C) HPLC chromatograph of InsP₆ and PP-InsPs in [³H]inositol-labeled S3^vector and S3^PPIP5K1 cells. (D) Cellular levels of InsP₆ and PP-InsPs, normalized to levels of [³H]inositol-lipids, from three independent experiments similar to that described in panel C.