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. 2018 Mar 16;38(2):BSR20171549. doi: 10.1042/BSR20171549

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© 2018 The Author(s).

This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

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(A) Silver-stained, SDS/PAGE analysis of recombinant PPIP5K1 purified from S3^PPIP5K1 cells, compared with S3^vector cells. (B) Western analysis of recombinant PPIP5K1. (C) Representative time course of 5-InsP₇ phosphorylation by 30 ng PPIP5K1 and just trace amounts of [³H]-labeled substrate (i.e under first-order conditions [24]). (D) Natural logarithmic transformation of the data in panel C, where [S]₀ = [³H]substrate at time zero, and [S]_t = [³H]substrate at time t, as indicated. (E) Time course of InsP₈ dephosphorylation by 220 ng recombinant PPIP5K1, determined by HPLC.