Skip to main content
. 2018 Mar 16;38(2):BSR20171549. doi: 10.1042/BSR20171549

Figure 6. Effects upon InsP8 phosphatase activity of large unilamellar vesicles containing either PtdIns(4,5)P2 or PtdIns(3,4,5)P3.

Figure 6

(A) Graphic depicting the assay; the incubation of LUVs with full-length PPIP5K1. The dark gray shading of four phospholipid head groups indicates that the concentration of the test polyphosphoinositide is 4.5%. (B) InsP8 phosphatase activities estimated from assays containing 100–150 ng of recombinant PPIP5K1, incubated for 35 min with LUVs containing 4.5% PtdIns(3,4,5)P3 or PtdIns(4,5)P2. Vertical bars depict means and SE for three experiments.