Table 1. Oligonucleotide sequences for qPCR analysis.
Gene symbol | Genbank ID | Forward primer | Reverse primer | Product size (bp) |
---|---|---|---|---|
Angptl3 | NM_001025065 | AAAGGGTTTTGGGAGGCTTGA | CCCAAAAGCGCTATGGTCTC | 117 |
Dgat1 | NM_053437 | GAACCGCTTCTTCCAAGGGA | AGAACTCCAGGCCCAGGTTA | 177 |
Dgat2 | NM_001012345 | ACCTACCTCGGATCTCGACC | CTGATCCATGCCCCAGCC | 105 |
Ephx2 | NM_022936 | CGTTCGACCTTGACGGAGTG | CTGGAAAGCGCCAAGTAGGA | 107 |
Nr1h3 | NM_031627 | GAGTCATCCGAGCCTACAGC | AAGAATCCCTTGCAGCCCTC | 191 |
Pparα | NM_013196 | ATTGGCGTTCGCAGCTGTTT | CTCGTGTGCCCTCCCTCAAG | 102 |
Srebf1 | NM_001276707 | CCATGGACGAGCTACCCTTC | GGCATCAAATAGGCCAGGGA | 149 |
β-actin | NM_031144 | ACTCTGTGTGGATTGGTGGC | CGCAGCTCAGTAACAGTCCG | 140 |
Abbreviations: Angptl3, angiopoietin-like 3; Dgat1, diacylglycerol O-acyltransferase 1; Dgat2, diacylglycerol O-acyltransferase 2; Ephx2, epoxide hydrolase 2; Nr1h3 (LXRα), nuclear receptor subfamily 1, group H, member 3; Pparα, peroxisome proliferator activated receptor α, Srebf1, sterol regulatory element binding transcription factor 1.