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. 2018 Mar 12;9:511. doi: 10.3389/fimmu.2018.00511

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Copyright © 2018 Zhang, Li, Li, Shen, Wang, Ji and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conformation specificity of the assay. (A) Native C-reactive protein (nCRP), monomeric CRP (mCRP), or recombinant mCRP (rmCRP) at the indicated concentrations were added to the indicated wells coated with 1:1,000 or 1:330 CRP-8 mAb, and the captured proteins were detected with a polyclonal CRP antibody from the BindingSite. (B) nCRP or mCRP at the indicated concentrations were added to wells (Thermo Fisher; catalog number: 442404) coated with 1:330 CRP-8 (clone 8) or 1:200 3H12 mAbs, and the captured proteins were detected with a polyclonal CRP antibody from the BindingSite.