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. 2018 Mar 12;9:511. doi: 10.3389/fimmu.2018.00511

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Copyright © 2018 Zhang, Li, Li, Shen, Wang, Ji and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adaptability of the assay. Monomeric CRP (mCRP) at the indicated concentrations in TBS buffer (A) or reference human sera (1:100) (B) were added to wells (Corning) coated with 1:1,000 CRP-8 mAb (clone 8), and the captured proteins were detected with a polyclonal CRP antibody from the BindingSite. The endogenous mCRP concentrations in the reference human sera were <10 ng/mL and therefore was undetectable following 1:100 dilution. The Pearson’s correlation coefficients (R) were also indicated.