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. 2018 Mar 12;9:469. doi: 10.3389/fimmu.2018.00469

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Copyright © 2018 Ling, Lua, Poh, Yeo, Lane and Gan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bubble chart representations of the production of recombinant Trastuzumab antibody variants. The production levels are directly proportional to the area of the circle. The columns show the Vκ family while the rows show the VH families. Each circle in the respective combination shows one of the three plasmid ratio transfections (red, 1:1; purple, 1:3; and green, 3:1) while black solid circles show the original pairing. All circles are benchmarked against the black solid circle which is at 100%. A table of the production rates is also shown in Figure S2 in Supplementary Material.