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. 2018 Mar 12;9:469. doi: 10.3389/fimmu.2018.00469

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Copyright © 2018 Ling, Lua, Poh, Yeo, Lane and Gan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The binding kinetics of recombinant Pertuzumab to Her2 and FcγIIa. The recombinant antibody variants are arranged according to VH families and color coded for Vκ (Vκ1—red, Vκ2—green, Vκ3—purple, Vκ4—blue, Vκ5—brown, and Vκ6—orange) while the original pairing is bolded with K_D, K_on, and K_off measurements shown. “Poor response” indicates that the antibody combinations did not yield reliable K_on and K_off during measurements (in triplicates) as determined by the Octet software. “Not produced” indicates that there was no useable amount of the purified product despite several transfection attempts.