Figure 5.

Stat3 and C/ebpβ influence FancC expression during emergency granulopoiesis. A, knockdown of Stat3 or C/ebpβ decreases IL1β-induced expression of Fanconi C. Murine bone marrow myeloid progenitor cells were transduced with vectors to express shRNAs specific for Stat3 or C/ebpβ (or scrambled control shRNA) and analyzed for FancC expression. Statistically significant differences are indicated by *, **, ***, #, ##, ###, or & (p < 0.01, n = 6 for all comparisons). B, expression of Stat3 and C/ebpβ increases early, Icsbp increases later, and FancC is increased throughout emergency granulopoiesis. Mice were injected with Alum (i.p.) to induce emergency granulopoiesis, and bone marrow Lin⁻CD34⁺ cells was collected 0, 1, 2, 3, or 4 weeks after injection. Gene expression was determined by quantitative real-time PCR. Statistically significant differences are indicated by *, **, ***, #, ##, ###, &, or && (p < 0.01, n = 6 for all comparisons).