Skip to main content
. 2018 Jan 29;293(11):3925–3936. doi: 10.1074/jbc.RA117.000425

Figure 5.

Figure 5.

PRMT7-mediated down-regulation of miR-221-3p and miR-221-5p levels is indispensable for the maintenance of mouse ESC stemness. A, schematic representation of the procedure for the treatment of cells with LNA–miR-221-3p and LNA–miR-221-5p. Cells were harvested 14 days after transfection of shPRMT7-7. B, microscopic and AP staining images of PRMT7-depleted V6.5 mouse ESCs. Mouse ESCs were treated with LNA-control, LNA–miR-221-5p or LNA–miR-221-3p on days 5, 7, 9, or 11 after transfection of shPRMT7-7. Red bars, 100 μm; BF, bright field; AP, alkaline phosphatase. C and D, analysis of mRNA levels of Oct4, Nanog, Sox2, c-Myc, and Klf4 after treatment of shLuc-transfected or PRMT7-depleted V6.5 mouse ESCs with LNA–miR-221-3p (C) or LNA–miR-221-5p (D) at different time points (days 5, 7, and 9). E, Western blot analysis of Oct4, Nanog, Sox2, Klf4, c-Myc, PRMT7, and β-actin (loading control) levels after treatment of shLuc-transfected or PRMT7-depleted V6.5 mouse ESCs with LNA-control, LNA–miR-221-3p or LNA–miR-221-5p. Data are presented as the mean ± S.D. of three independent experiments. *, p < 0.05; **, p < 0.01; and ***, p < 0.001.