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. 2018 Feb 5;293(11):3981–3988. doi: 10.1074/jbc.RA117.000885

Figure 1.

Figure 1.

MAR1 ameliorates palmitate-induced TG accumulation and expression of lipogenic genes in primary hepatocytes. A, Oil Red-O staining in hepatocytes in the presence of 200 μm palmitate and MAR1 (0–10 μm) for 24 h. TG accumulation was quantitated by isopropyl alcohol extraction. Western blot analysis of SREBP1 (processed), FAS, and SCD1 expression (B) and quantitative RT-PCR of Gpat1, Me, Mgat, and Dgat1 mRNA expression (C) in hepatocytes in the presence of 200 μm palmitate and MAR1 (0–10 μm) are shown. D, Western blot analysis and quantitative RT-PCR of ER stress markers in hepatocytes in the presence of 200 μm palmitate and MAR1 (0–10 μm) for 24 h. Means ± S.D. were calculated from three independent experiments. One-way ANOVA with Tukey post hoc was performed. ***, p < 0.001 and **, p < 0.01 when compared with expression levels in the controls. !!!, p < 0.001, !!, p < 0.01, and !, p < 0.05 when compared with palmitate treatment.