Skip to main content
. 2018 Feb 5;293(11):3981–3988. doi: 10.1074/jbc.RA117.000885

Figure 2.

Figure 2.

AMPK contributes to the effects of MAR1 on palmitate-induced TG accumulation and ER stress. A, Western blot analysis and quantitative RT-PCR of ER stress markers in hepatocytes in the presence of 200 μm palmitate and MAR1 (0–10 μm) for 24 h. Western blot analysis of ER stress markers' phosphorylation and expression and quantitative RT-PCR of spliced Xbp-1 mRNA expression (B) and Oil Red-O staining (C) in transfected hepatocytes with sequence-scrambled siRNA or AMPK siRNA in the presence of 200 μm palmitate and MAR1 (10 μm) for 24 h are shown. TG accumulation was quantitated by a TG assay kit. Western blot analysis (D) and quantitative RT-PCR (E) of lipogenic markers in transfected hepatocytes with sequence-scrambled siRNA or AMPK siRNA in the presence of 200 μm palmitate and MAR1 (10 μm) for 24 h are shown. Means ± S.D. were calculated from three independent experiments. One-way ANOVA with Tukey post hoc was performed. ***, p < 0.001 and **, p < 0.01 when compared with expression levels in control or sequence-scrambled siRNA. !!!, p < 0.001 and !!, p < 0.01 when compared with palmitate treatment. ###, p < 0.001 and #, p < 0.05 when compared with palmitate plus MAR1 treatment.