Figure 3.

Overexpression of Gm10768 increases gluconeogenic gene expression in vitro. Mouse primary hepatocytes were isolated from six mice to do one batch of experiments and transduced with adenoviruses expressing Gm10768 (Ad-Gm10768) or GFP (Ad-GFP, as control) for 48 h, and three batches of experiments were performed. RT-qPCR (A) and Western blot analysis (B) were performed to check gluconeogenic gene expression. *, p < 0.05, and **, p < 0.01 versus Ad-GFP, unpaired t test. Data were presented as mean ± S.D. Error bars represent the S.D. from the mean of three independent experiments.