Figure 4.

Overexpression of Gm10768 promotes hepatic gluconeogenesis in vivo. C57BL/6J mice were transduced with adenoviruses encoding GFP or Gm10768 through tail-vein injection (1 × 10⁹ PFU per mouse). Four days later, animal experiments were performed (n = 6 per group). A, infection efficiency of Ad-Gm10768 in the liver of normal C57BL/6J mice. B–D, GTT, ITT, and PTT. Mice were fasted for 6 h (for GTT and ITT) or overnight (for PTT), and the assays were performed. Area under curve (AUC) for these tests were calculated and shown simultaneously. E and F, gluconeogenic gene expression in the liver. RT-qPCR (E) and Western blot analysis (F) were performed in the liver of mice treated as in A–D. *, p < 0.05, and **, p < 0.01 versus Ad-GFP, unpaired t tests. Data were presented as mean ± S.D. Error bars represent the S.D. from the mean of three independent experiments.