Figure 6.

Kinetics of [¹⁴C]aspartate or [¹⁴C]malate uptake by AtUCP1- and AtUCP2-reconstituted liposomes containing no substrate or various substrates. Proteoliposomes containing AtUCP1 (A and C) or AtUCP2 (B and D) were preloaded internally with 10 mm aspartate (●), cysteate (▴), d-aspartate (♦), or 10 mm NaCl and no substrate (▾) (A and B) and with malate (▵), oxaloacetate (♦), malonate (●), succinate (▴), phosphate (■), aspartate (□), or 10 mm NaCl and no substrate (▾) (C and D). Transport was initiated by adding 1 mm [¹⁴C]aspartate (A and B) or 1 mm [¹⁴C]malate (C and D) and terminated at the indicated times. Similar results were obtained in at least three independent experiments.