Figure 7.

Efflux of [¹⁴C]aspartate and [¹⁴C]malate from AtUCP1- and AtUCP2-reconstituted liposomes. Proteoliposomes containing AtUCP1 (A and C) and AtUCP2 (B and D) with 5 mm aspartate and 5 mm malate internally were loaded with [¹⁴C]aspartate and [¹⁴C]malate, respectively, by carrier-mediated exchange equilibrium, and external substrate was removed by Sephadex G-75. Efflux of [¹⁴C]aspartate (A and B) and [¹⁴C]malate (C and D) was started by adding 5 mm aspartate (●), malate (▵), glutamate (○), 5 mm NaCl and no substrate (▾), and 5 mm aspartate (A and B) or 5 mm malate (C and D) together with 20 mm pyridoxal 5′-phosphate and 20 mm bathophenanthroline (♢). The transport was terminated at the indicated times. Similar results were obtained in at least three independent experiments.