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Analysis of the interactions of TolC with gp44 in vitro. Western blot analyses of GST pulldown experiments were performed with GST-TolC immobilized on glutathione-Sepharose resin and His-tagged protein extracts of full-length gp44 (lane 1), gp44(451–753) (lane 2), and gp44(1–450) (lane 3); the protein mixture (full-length gp44, gp44(451–753) and gp44(1–450)) described above was incubated with GST for pulldown analysis as a negative control (lane 4). Lane M indicates mass.
