Figure 8.

Sequence alignments of TolC of E. coli and V. cholerae and the predicted motifs of VCA0781. A, comparison of TolC sequence of V. cholerae and E. coli using program Clustal Omega. Identical amino acids are labeled in dark gray; similar amino acids are colored in light gray. The locations of the two loops of TolC (E. coli) are labeled with a brace. Sites Ala-78 and Gly-290–Glu-291 of TolC (V. cholerae) are marked with black box. B, predicted domains of VCA0781 based on the comparison with MacB of E. coli. The domains of VCA0781 are illustrated with the square frames, and the start and end amino acid positions are marked.