Table 3.
Sensitivity of strains to VP3 detected by double-layer plaque assay
The following symbols and abbreviation were used: +, indicates strain with no plaque formation confirmed as VP3-resistant phenotype; −, indicates strain with plaque formation confirmed as VP3-sensitive phenotype; PC, N16961 (wildtype) without VP3 used as positive control (PC); NC, N16961 (wildtype) with VP3 used as negative control (NC).
| Strains | Growth rate (A600) | Double-layer plaque assay | Strains | Growth rate (A600) | Double-layer plaque assay |
|---|---|---|---|---|---|
| PC | 0.880 | + | NΔtolC-OS | 0.921 | + |
| NC | 0.021 | − | NΔtolC-OS-C | 0.017 | − |
| NΔOS | 0.889 | + | NΔtolC-OS-CtolC | 0.888 | + |
| NΔOS-C | 0.016 | − | NΔtolC-OS-COS | 0.904 | + |
| NΔtolC | 0.865 | + | NΔtolC-C | 0.021 | − |